Journal
FEBS LETTERS
Volume 586, Issue 7, Pages 990-995Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2011.12.040
Keywords
Bm61; Bombyx mori nucleopolyhedrovirus; BmNPV; Gene deletion
Funding
- National Basic Research Program of China [2012CB114604]
- Natural Science Foundation of China [31101673, 30871826]
- Senior Talent Foundation of Jiangsu University [1281270014]
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All lepidopteran baculovirus genomes sequenced encode a homolog of the Bombyx mori nucleopolyhedrovirus orf61 gene (Bm61). To determine the role of Bm61 in the baculoviral life cycle, we constructed a Bm61 knockout virus and characterized it in cells. We observed that the Bm61 deletion bacmid led to a defect in production of infectious budded virus (BV). Quantitative PCR analysis of BV in the media culturing the transfected cell indicated that BV was not produced due to Bm61 deletion. Electron microscope analysis showed that in the knockout of Bm61, nucleocapsids were not transported from the nucleus to the cytoplasm. From these results we concluded that BM61 is required in the BV pathway for the egress of nucleocapsids from the nucleus to the cytoplasm. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
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