Journal
FEBS LETTERS
Volume 586, Issue 6, Pages 668-674Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2012.01.058
Keywords
Fluorescence correlation spectroscopy (FCS); Fluorescence cross-correlation spectroscopy (FCCS); Transporting vesicle (TV); Glycophorin C (GPC); Protein 4.1R (4.1R)
Funding
- Japan Society for the Promotion of Science [22790531]
- Grants-in-Aid for Scientific Research [22790531] Funding Source: KAKEN
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Interaction of protein 4.1 (4.1R) with the transmembrane protein glycophorin C (GPC) regulates the functions of erythrocyte membrane. Fluorescence correlation spectroscopy (FCS) was used to define the interaction of EGFP-4.1R with DsRed-GPC on transport vesicles (TVs) by measuring their fluctuation in living cells. DsRed-GPC expressed in HeLa cells was delivered to the plasma membrane through slow vesicle transport. EGFP-4.1R, which freely diffused in the cytosol when expressed alone, diffused slowly when co-expressed with DsRed-GPC, indicating association of EGFP-4.1R with TVs. Fluorescence cross-correlation spectroscopy (FCCS) showed direct interaction of EGFP-4.1R with DsRed-GPC on TVs. The present study demonstrates that 4.1R binds to GPC on TVs in living cells. Structured summary of protein interactions: GPC and 4.1R30 physically interact by fluorescence correlation spectroscopy (View interaction) (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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