Journal
FEBS LETTERS
Volume 587, Issue 2, Pages 220-225Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2012.11.029
Keywords
Ferroxidase center; Ferritin; Phosphate; Displacement; Bacteria; Archaea
Funding
- Dutch National Research School Combination, Catalysis Controlled by Chemical Design (NRSC-Catalysis)
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The iron-storage protein, ferritin, is widely found in all Domains of life. A conserved diiron center in ferritin catalyzes oxidation of Fe(II) and regulates storage of the resultant Fe(III) oxidation product. When this center is filled with Fe(III), in bacterial or archaeal ferritin the presence of phosphate accelerates the rate of Fe(II) oxidation. The molecular mechanism underlying this stimulatory effect of phosphate is unknown. Using site directed mutagenesis of the residues in the diiron center of the archaeal ferritin from Pyrococcus furiosus we show that phosphate facilitates displacement of Fe(III) by Fe(II) from this site. Therefore, the rate of Fe(II) oxidation increases only when the ferroxidase center is filled with Fe(III). (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
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