Journal
FEBS LETTERS
Volume 586, Issue 16, Pages 2375-2381Publisher
WILEY
DOI: 10.1016/j.febslet.2012.05.049
Keywords
miR-100; Osteogenesis; hASCs; BMPR2
Funding
- 863 Projects'' of Ministry of Science and Technology of PR China [2011AA020100]
- National Natural Science Foundation of China [30830052]
- National Key Scientific Program of China [2011CB964901]
- Program for Cheung Kong Scholars and Innovative Research Team in University-PCSIRT [IRT0909]
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Elucidation of the molecular mechanisms governing human adipose-derived mesenchymal stem cells (hASCs) osteogenic differentiation is of great importance for improving the treatment of bone-related diseases. In this study, we examined the role of microRNA (miR)-100 on the osteogenesis of hASCs. Overexpression of miR-100 inhibited osteogenic differentiation of hASCs in vitro, whereas downregulation of miR-100 enhanced the process. Target prediction analysis and dual luciferase report assay confirmed that bone morphogenetic protein receptor type II (BMPR2) was a direct target of miR-100. Furthermore, knockdown of BMPR2 by RNA interference inhibited osteogenic differentiation of hASCs, similar as the effect of upregulation miR-100. Taken together, our findings imply that miR-100 plays a negative role in osteogenic differentiation and might act through targeting BMPR2. (c) 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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