Journal
FEBS LETTERS
Volume 585, Issue 19, Pages 2998-3005Publisher
WILEY
DOI: 10.1016/j.febslet.2011.08.014
Keywords
Ras homolog gene family, member B; miR-21; Colorectal cancer; Small G protein
Funding
- National Natural Science Foundation of China [81000877]
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It has become increasingly clear that microRNAs play an important role in many human diseases including cancer. Here, we show that expression of miR-21 in HEK293 and several colorectal cancer cells was found inversely correlated with ras homolog gene family, member B (RhoB) expression. miR-21 expression significantly suppressed RhoB 3' UTR luciferase-reporter activity, but the inhibitory effect was lost when the putative target sites were mutated. Exogenous miR-21 over-expression mimicked the effect of RhoB knockdown in promoting proliferation and invasion and inhibiting apoptosis, whereas anti-miR-21 or RhoB expression yielded opposite effects, in colorectal cancer cells. These results suggest that miR-21 is a regulator of RhoB expression and RhoB could be a useful target in exploring the potential therapeutic benefits of miR-21 mediated tumor cell behaviors in colorectal cancer. (C) 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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