Journal
FEBS LETTERS
Volume 585, Issue 7, Pages 1061-1066Publisher
WILEY
DOI: 10.1016/j.febslet.2011.03.012
Keywords
Antibiotic resistance; Flavin; Tigecycline; Tetracycline degradation; TetX
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The flavin-dependent monooxygenase TetX confers resistance to all clinically relevant tetracyclines, including the recently approved, broad-spectrum antibiotic tigecycline (Tygacil (R)) which is a critical last-ditch defense against multidrug-resistant pathogens. TetX represents the first resistance mechanism against tigecycline, which circumvents both the tet-gene encoded resistances, relying on active efflux of tetracyclines, and ribosomal protection proteins. The alternative enzyme-based mechanism of TetX depends on regioselective hydroxylation of tetracycline antibiotics to 11a-hydroxy-tetracyclines. Here, we report the X-ray crystallographic structure determinations at 2.1 angstrom resolution of native TetX from Bacteroides thetaiotaomicron and its complexes with tetracyclines. Our crystal structures explain the extremely versatile substrate diversity of the enzyme and provide a first step towards the rational design of novel tetracycline derivatives to counter TetX-based resistance prior to emerging clinical observations. (C) 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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