Journal
FEBS LETTERS
Volume 585, Issue 12, Pages 1905-1909Publisher
WILEY
DOI: 10.1016/j.febslet.2011.04.070
Keywords
Thioredoxin 2; Methionine sulfoxide reductase; Kinetic parameter; Complementation; Escherichia coli
Funding
- Centre National de la Recherche Scientifique
- Nancy Universite
- Institut Federatif de Recherches [111]
- PRST Bioingenierie
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Thioredoxins (Trx) 1 and 2, and three methionine sulfoxide reductases (Msr) whose activities are Trx-dependent, are expressed in Escherichia coli. A metB(1) trxA mutant was shown to be unable to grow on methionine sulfoxide (Met-O) suggesting that Trx2 is not essential in the Msr-recycling process. In the present study, we have determined the kinetic parameters of the recycling process of the three Msrs by Trx2 and the in vivo expression of Trx2 in a metB(1) trxA mutant. The data demonstrate that the lack of growth of the metB(1) trxA mutant on Met-O is due to low in vivo expression of Trx2 and not to the lower catalytic efficiency of Msrs for Trx2. (C) 2011 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
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