Journal
FEBS LETTERS
Volume 585, Issue 14, Pages 2339-2344Publisher
WILEY
DOI: 10.1016/j.febslet.2011.06.002
Keywords
Subsite affinities; Endo-activity; Product displacement; Family 18 chitinases
Funding
- Norwegian Research Council [164653/V40, 140497/I30, 177542/V30]
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The contributions of the -3 subsite and a putative +3 subsite to substrate positioning in ChiA from Serratia marcescens have been investigated by comparing how ChiA and its -3 subsite mutant W167A interact with soluble substrates. The data show that Trp - GlcNAc stacking in the -3 subsite rigidifies the protein backbone supporting the formation of the intermolecular interaction network that is necessary for the recognition and positioning of the N-acetyl groups before the -1 subsite. The +3 subsite exhibits considerable substrate affinity that may promote endo-activity in ChiA and/or assist in expelling dimeric products from the +1 and +2 subsites during processive hydrolysis. (C) 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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