Journal
FEBS LETTERS
Volume 586, Issue 2, Pages 174-179Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2011.12.012
Keywords
ComX pheromone; Posttranslational modification; Prenylation; Prenyltransferase; Quorum sensing; Bacillus subtilis
Funding
- Ministry of Education, Culture, Sports, Science and Technology of Japan [212118, 18101009]
- Grants-in-Aid for Scientific Research [18101009, 09J01128, 23658098, 09J01228] Funding Source: KAKEN
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ComX pheromone is an isoprenoidal oligopeptide containing a modified tryptophan residue, which stimulates natural genetic competence in the gram-positive bacterium Bacillus. Since posttranslational prenylation on the tryptophan residue has not been reported except in ComX pheromone, the universality of this modification has not yet been elucidated. In this paper, we established a cell-free system, whereby the tryptophan residue in peptides is modified with a geranyl group by modifying enzyme ComQ. In addition, we investigated enzymatic reaction conditions using an in vitro enzyme reaction system. This is the first report of in vitro geranylation on the tryptophan residue. This system is potentially a useful tool for elucidating the universality of prenylation on the tryptophan residue. (C) 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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