Journal
FEBS LETTERS
Volume 584, Issue 4, Pages 689-693Publisher
WILEY
DOI: 10.1016/j.febslet.2010.01.022
Keywords
Chromodomain, helicase, DNA-binding protein 8 (CHD8); HOX gene regulation; ATP-dependent chromatin remodeling; WDR5
Funding
- American Cancer Society Research [RSG-07-18901]
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Chromodomain, helicase, DNA-binding protein 8 (CHD8) is an ATP-dependent chromatin remodeling enzyme that has been demonstrated to exist within a large protein complex which includes WDR5, Ash2L, and RbBP5, members of the Mixed Lineage Leukemia (MLL) histone modifying complexes. Here we show that CHD8 relocalizes to the promoter of the MLL regulated gene HOXA2 upon gene activation. Depletion of CHD8 enhances HOXA2 expression under activating conditions. Furthermore, depletion of CHD8 results in a loss of the WDR5/Ash2L/RbBP5 subcomplex, and consequently H3K4 trimethylation, at the HOXA2 promoter. These studies suggest that CHD8 alters HOXA2 gene expression and regulates the recruitment of chromatin modifying enzymes.
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