Journal
FEBS LETTERS
Volume 584, Issue 18, Pages 4048-4052Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2010.08.025
Keywords
MicroRNA; miR-542-3p; Cell cycle arrest; Survivin; 18S ribosomal RNA
Funding
- Korea government (MEST) [2009-0075381]
- National Research Foundation of Korea [2009-0075381] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Survivin is a protein which functions as a mitotic regulator as well as apoptosis inhibitor. In this study, we show that introduction of synthetic miR-542-3p mimetic reduced both mRNA and protein levels of survivin. In A549 cells, luciferase reporter assay revealed that miR-542-3p targeted predicted binding sites in the 3'-untranslated region (3'-UTR) of survivin. We also demonstrate that ectopic expression of miR-542-3p inhibited cell proliferation by inducing Gap 1 (G1) and Gap 2/Mitosis (G2/M) cell cycle arrest. Collectively, these results suggest that survivin is a direct target of miR-542-3p and growth inhibition by miR-542-3p may have a potential utility as an anti-cancer therapy. (c) 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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