Journal
FEBS LETTERS
Volume 584, Issue 5, Pages 995-1000Publisher
WILEY
DOI: 10.1016/j.febslet.2010.01.044
Keywords
TNF-alpha; Insulin resistance; HepG2; Reactive oxygen species; NADPH oxidase 3
Funding
- National Basic Research Program of China [2006CB503910]
- National High-Tech R&D Program of China [2006AA02A408]
- National Natural Science foundation of China [30572082]
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TNF-alpha-induced insulin resistance is associated with generation of reactive oxygen species (ROS). This study aims at defining the link between ROS production and hepatic insulin resistance. Treatment with TNF-alpha increased ROS generation through activating NADPH oxidase 3 (NOX3) in HepG2 hepatocytes. Down-regulation of NOX3 using siRNA prevented TNF-alpha-induced decrease of cellular glycogen. In the cells treated with TNF-alpha, there were NOX3-dependent activation of JNK, inhibition of IRS1 and phosphorylation of AKT/PKB and GSK. In conclusion, the effects of TNF-alpha on hepatic insulin resistance appear to be, at least in part, mediated by NOX3-derived ROS through a JNK pathway. (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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