Journal
FEBS LETTERS
Volume 585, Issue 1, Pages 183-186Publisher
WILEY
DOI: 10.1016/j.febslet.2010.11.037
Keywords
gun mutants; cch; Synechocystis; Arabidopsis thaliana; Magnesium chelatase
Funding
- BBSRC
- BBSRC [BB/G021546/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/G021546/1] Funding Source: researchfish
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The chlorophyll-deficient gun5-1 and cch Arabidopsis mutants carry single point mutations in the CHLH subunit of the magnesium chelatase enzyme, which catalyses the first committed step of chlorophyll biosynthesis. Recombinant Synechocystis ChlH subunits carrying the gun5-1 or cch mutations are inactive in Mg-chelatase assays, despite being able to bind both substrate and product, and retaining a capacity to form a ChlH-ChlI-ChlD Mg-chelatase complex. These mutant subunits act as inhibitors of ChlH, showing that the ChlH-porphyrin complex associates reversibly with the ChlI and D subunits during the catalytic cycle. This inhibition is reversed upon addition of Gun4. (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
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