Journal
FEBS LETTERS
Volume 584, Issue 13, Pages 2759-2766Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2010.04.062
Keywords
Enzyme catalysis; Tunneling; Barrier compression; Promoting mode; Kinetic isotope effect; Catalytic entropy
Funding
- National Institutes of Health (NIH) [GM024492]
- Slovenian Research Agency [J1-2014]
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The idea that tunneling is enhanced by the compression of the donor-acceptor distance has attracted significant interest. In particular, recent studies argued that this proposal is consistent with pressure effects on enzymatic reactions, and that the observed pressure effects support the idea of vibrationally enhanced catalysis. However, a careful analysis of the current works reveals serious inconsistencies in the evidence presented to support these hypotheses. Apparently, tunneling decreases upon compression, and external pressure does not lead to the applicable compression of the free energy surface. Additionally, pressure experiments do not provide actual evidence for vibrationally enhanced catalysis. Finally, the temperature dependence of the entropy change in hydride transfer reactions is shown to reflect simple electrostatic effects. (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
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