Journal
FEBS LETTERS
Volume 584, Issue 8, Pages 1553-1557Publisher
WILEY
DOI: 10.1016/j.febslet.2010.03.009
Keywords
DNA repair; DNA glycosylase; Endonuclease VIII homolog; 8-Oxoadenine
Funding
- RAS Presidium [22.14]
- RFBR [08-04-00596]
- Russian Ministry of Education and Science [02.740.11.0079/NSh-652.2008.4]
- Medical Research Council
- Cancer Research UK
- Cancer Research UK [9194] Funding Source: researchfish
- Medical Research Council [G0700730] Funding Source: researchfish
- MRC [G0700730] Funding Source: UKRI
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8-oxo-7,8-dihydroadenine (8-oxoAde) is a major product of adenine modification by reactive oxygen species. So far, only one mammalian DNA glycosylase, 8-oxoguanine-DNA-glycosylase 1 (OGG1), has been shown to excise 8-oxoAde, exclusively from pairs with Cyt. We have found that endonuclease VIII-like protein 1 (NEIL1), a mammalian homolog of bacterial endonuclease VIII, can efficiently remove 8-oxoAde from 8-oxoAde: Cyt pairs but not from other contexts. In an in vitro reconstituted system, reactions containing OGG1 produced a fully repaired product, whereas NEIL1 caused an abortive initiation of repair, stopping after 8-oxoAde removal and DNA strand cleavage. This block was partially relieved by polynucleotide kinase/30-phosphatase. Thus, two alternative routes of 8-oxoAde repair may exist in mammals. (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
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