Journal
FEBS LETTERS
Volume 584, Issue 5, Pages 1016-1020Publisher
WILEY
DOI: 10.1016/j.febslet.2010.01.055
Keywords
Apoptosis; Cationic liposome; Macrophage; PKC delta; Reactive oxygen species
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We have recently demonstrated that reactive oxygen species (ROS) play an important role in RAW264.7 cell apoptosis induced by cationic liposomes composed of stearylamine (SA-liposomes). In this study, we investigated whether protein kinase C delta PKC delta) is involved in apoptosis induced by cationic liposomes. Tyrosine phosphorylation, nuclear localization, and cleavage of PKCd were observed following the treatment of cells with SA-liposomes, suggesting that SA-liposomes activate PKCd. Rottlerin, a specific inhibitor of PKCd, inhibited ROS generation and also suppressed apoptosis. Cell surface proteoglycans may contribute to PKCd activation by SA-liposomes. These findings suggest that PKCd is strongly associated with apoptosis induced by SA-liposomes. (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
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