Journal
FEBS LETTERS
Volume 584, Issue 6, Pages 1085-1090Publisher
WILEY
DOI: 10.1016/j.febslet.2010.02.031
Keywords
CCDC106; p53; Variant transcript; Nuclear localisation signal; Protein degradation
Funding
- National Basic Research Program of China [2008CB517306, 2005CB522505]
- Scientific Research Fund of Hunan Provincial Education Department [09A052, 09B059]
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The putative CCDC106 protein was previously identified as a p53-interacting partner by automated yeast two-hybrid screening, but its sequence and function have not been validated experimentally. Here, we identified three variant transcripts of the CCDC106 gene; these transcripts differ in their second exons due to the use of different splice acceptor site, but encode an identical protein of 280 residues. A bipartite nuclear localisation signal at residues 151-164 mediates the nuclear localisation of CCDC106. Double immunofluorescence staining revealed the colocalisation of endogenous CCDC106 and p53 protein in nuclei. The in vivo interaction between CCDC106 and p53 was confirmed by a co-immunoprecipitation assay. Furthermore, we demonstrated that CCDC106 promotes the degradation of p53 protein and inhibits its transactivity. Structured summary: MINT-7681390: CCDC106 (uniprotkb: Q9BWC9) physically interacts (MI: 0915) with p53 ( uniprotkb: P04637) by anti tag co-immunoprecipitation ( MI: 0007) MINT-7681212: p53 ( uniprotkb: P04637) and CCDC106 ( uniprotkb: Q9BWC9) colocalise ( MI: 0403) by fluorescence microscopy ( MI: 0416) (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
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