Journal
FEBS LETTERS
Volume 584, Issue 13, Pages 2937-2941Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2010.05.022
Keywords
D-Serine; NMDA receptor; Glutamate; Gliotransmission; Serine racemase
Funding
- Israel Science Foundation
- NARSAD
- ISF-Legacy Heritage Fund
- Ministry of Health
- EU
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Serine racemase (SR) catalyses the synthesis of the transmitter/neuromodulator D-serine, which plays a major role in synaptic plasticity and N-methyl D-aspartate receptor neurotoxicity. We now report that SR is phosphorylated at Thr71 and Thr227 as revealed by mass spectrometric analysis and in vivo phosphorylation assays. Thr71 phosphorylation was observed in the cytosolic and membrane- bound SR while Thr227 phosphorylation was restricted to the membrane fraction. The Thr71 site has a motif for proline-directed kinases and is the main phosphorylation site of SR. Experiments with a phosphorylation-deficient SR mutant indicate that Thr71 phosphorylation increases SR activity, suggesting a novel mechanism for regulating D-serine production. (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
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