Journal
FEBS LETTERS
Volume 584, Issue 14, Pages 3143-3148Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2010.05.057
Keywords
Reactive oxygen species; Heat stress; Substrate oxidation; Modular kinetic analysis; Coupling efficiency; Membrane potential (Delta Psi)
Funding
- Ministry of Education, Science, and Culture of Japan [21380165]
- Japan Society for the Promotion of Science (JSPS) for Young Scientists [200807429]
- Tohoku University Institute for International Advanced Research and Education
- Grants-in-Aid for Scientific Research [21380165, 22790841] Funding Source: KAKEN
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We previously showed that heat stress stimulates reactive oxygen species (ROS) production in skeletal muscle mitochondria of birds, probably via an elevation in mitochondrial membrane potential (Delta Psi). To clarify the mechanism underlying the elevation of Delta Psi, modular kinetic analysis was applied to oxidative phosphorylation in skeletal muscle mitochondria of heat-stressed birds (34 degrees C for 12 h). In the birds exposed to heat stress, 'substrate oxidation' (a Delta Psi-producer) was increased compared to control (24 degrees C) birds, although there was little difference in 'proton leak' (a Delta Psi-consumer), suggesting that an elevation in the Delta Psi at state 4 may be due to enhanced substrate oxidation. It thus appears that enhanced substrate oxidation plays a crucial role in the overproduction of ROS for heat-stressed birds, probably via elevated Delta Psi. (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
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