Journal
FEBS LETTERS
Volume 583, Issue 5, Pages 897-902Publisher
WILEY
DOI: 10.1016/j.febslet.2009.01.048
Keywords
Prominin; CD133; Cholesterol; Microvilli; Epithelial cell; Cancer
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We previously reported on the occurrence of prominin-1-carrying membrane vesicles that are released into body fluids from microvilli of epithelial cells. This release has been implicated in cell differentiation. Here we have characterized these vesicles released from the differentiated Caco-2 cells. We find that in these vesicles, prominin-1 directly interacts with membrane cholesterol and is associated with a membrane microdomain. The cholesterol depletion using methyl-beta-cyclodextrin resulted in a marked increase in their release, and a dramatic change in the microvillar ultrastructure from a tubular shape to a pearling state, with multiple membrane constrictions, suggesting a role of membrane cholesterol in vesicle release from microvilli. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
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