Journal
FEBS LETTERS
Volume 584, Issue 1, Pages 135-140Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2009.11.030
Keywords
Endothelial cell; mTOR; S6K1; Thrombin; Tissue factor; TNF alpha
Funding
- Swiss National Science Foundation [310000-120435/1]
- Swiss Heart Foundation
- Swiss Cardiovascular Research and Training Network (SCRTN) Programme
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Rapamycin has been reported to enhance tissue factor (TF) expression. The present study investigated roles of mammalian target of rapamycin (mTOR) and its downstream S6K1 in this process. We showed here that, consistent with rapamycin, knocking-down mTOR enhanced thrombin-induced TF mRNA and protein levels, whereas silencing S6K1 mitigated up-regulation of TF protein but not TF mRNA level. The enhanced TF protein level upon mTOR-silencing was further augmented by over-expression of a constitutively active S6K1 mutant and reduced by blocking RhoA, p38(mapk) or NF-kappa B. The results reveal an opposing and uncoupling effect of mTOR and S6K1 in regulating TF expression. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
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