Journal
FEBS LETTERS
Volume 584, Issue 1, Pages 27-32Publisher
WILEY
DOI: 10.1016/j.febslet.2009.10.090
Keywords
Src; Escherichia coli K1; Human brain microvascular endothelial cell; Phosphatidylinositol 3-kinase; Blood-brain barrier
Funding
- National Natural Science Foundation of China [30570094, 30500277, 30600327, 30840013]
- Innovation Team Program Foundation of Liaoning Province [2006T131]
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Invasion of brain microvascular endothelial cells is a prerequisite for successful crossing of the blood-brain barrier by Escherichia coli (E. coli), but the underlying mechanism remains unclear. Here we showed activation of Src tyrosine kinase in E. coli K1 invasion of human brain microvascular endothelial cells (HBMEC). E. coli invasion of HBMEC and the E. coli-induced rearrangement of actin. laments were blocked by Src inhibitors. Overexpression of dominant-negative Src in HBMEC significantly attenuated E. coli invasion and the concomitant actin. laments rearrangement. Furthermore, E. coli K1-triggered phosphatidylinositol 3-kinase (PI3K) activation in HBMEC was effectively blocked by Src inhibitors and dominant-negative Src. These results demonstrated the involvement of Src and its interaction with PI3K in E. coli K1 invasion of HBMEC.
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