Journal
FEBS LETTERS
Volume 583, Issue 7, Pages 1207-1214Publisher
WILEY
DOI: 10.1016/j.febslet.2009.03.011
Keywords
Human cytomegalovirus; Brefeldin A; Viral replication; de novo DNA synthesis; Protein expression; Capsid formation
Funding
- Wilhelm Sander Foundation [2004.031.2]
- Deutsche Forschungsgemeinschaft [1214/5-4]
- Sonnenfeld-Foundation Berlin
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In this study we used the fungal antibiotic brefeldin A (BFA) to analyze its effect on viral replication. Analysis by electron microscopy demonstrated that no viral particles were observed in cells treated before the onset of viral replication. In the presence of BFA expression of IE2, MCP, pUL104, pUL56 and pUL89 were reduced, while no or slight effect was observed on expression of pp65, pUL44 and pUL57. Strikingly, real time PCR revealed that de novo viral DNA synthesis is reduced but not completely abolished in the presence of BFA. These results indicated that BFA represents a multi-functional compound leading to inhibition of several steps of viral maturation such as expression of viral DNA packaging proteins and capsid formation. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
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