Journal
FEBS LETTERS
Volume 583, Issue 17, Pages 2839-2842Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2009.07.041
Keywords
CALI; Fluorescent proteins; X-ray crystallography; Reactive oxygen species; Solvent channel
Funding
- CEA
- ANR [ANR-07-BLAN-0107-01, ANR06-PCVI-0022-02]
Ask authors/readers for more resources
The red fluorescent protein KillerRed, engineered from the hydrozoan chromoprotein anm2CP, has been reported to induce strong cytotoxicity through the chromophore assisted light inactivation (CALI) effect. Here, we present the X-ray structures of KillerRed in its native and bleached states. A long water-filled channel is revealed, connecting the methylene bridge of the chromophore to the solvent. This channel facilitates the transit of oxygen and of reactive oxygen species (ROS) formed by reaction with the excited chromophore. The functional roles of key mutations used to produce KillerRed are discussed, strong chromophore distortions in the bleached state are revealed, and mechanisms for ROS production and self protection are proposed. The presence of a partially mature, photo-resistant, green-emitting state is characterized, which accounts for enhanced CALI by pre-bleached KillerRed. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available