Journal
FEBS LETTERS
Volume 583, Issue 3, Pages 526-530Publisher
WILEY
DOI: 10.1016/j.febslet.2009.01.019
Keywords
CD; Crosslinking; LOV domain; Oligomeric structure; Phototropin; Arabidopsis
Funding
- Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan [15076210, 13139205]
- Grants-in-Aid for Scientific Research [13139205] Funding Source: KAKEN
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Oligomeric structures of the four LOV domains in Arabidopsis phototropin1 (phot1) and 2 (phot2) were studied using crosslinking. Both LOV1 domains of phot1 and phot2 form a dimer independently on the light conditions, suggesting that the LOV1 domain can be a stable dimerization site of phot in vivo. In contrast, phot1-LOV2 is in a monomer-dimer equilibrium and phot2-LOV2 exists as a monomer in the dark. Blue light-induced a slight increase in the monomer population in phot1-LOV2, suggesting a possible blue light-inducible dissociation of dimers. Furthermore, blue light caused a band shift of the phot2-LOV2 monomer. CD spectra revealed the unfolding of helices and the formation of strand structures. Both light-induced changes were reversible in the dark.
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