Journal
FEBS LETTERS
Volume 583, Issue 22, Pages 3690-3696Publisher
WILEY
DOI: 10.1016/j.febslet.2009.10.057
Keywords
Thylakoid; Hcf106; Photobleaching; Tat system; Photosystem II
Funding
- Biotechnology and Biological Sciences Research Council
- Engineering and Physical Sciences Research Council
- Biotechnology and Biological Sciences Research Council [BB/C00437X/1, SF14949] Funding Source: researchfish
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The thylakoid membrane forms stacked thylakoids interconnected by 'stromal' lamellae. Little is known about the mobility of proteins within this system. We studied a stromal lamellae protein, Hcf106, by targeting an Hcf106-GFP fusion protein to the thylakoids and photobleaching. We find that even small regions fail to recover Hcf106-GFP fluorescence over periods of up to 3 min after photobleaching. The protein is thus either immobile within the thylakoid membrane, or its diffusion is tightly restricted within distinct regions. Autofluorescence from the photosystem II light-harvesting complex in the granal stacks likewise fails to recover. Integral membrane proteins within both the stromal and granal membranes are therefore highly constrained, possibly forming 'microdomains' that are sharply separated. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
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