Journal
FEBS LETTERS
Volume 584, Issue 1, Pages 233-238Publisher
WILEY
DOI: 10.1016/j.febslet.2009.11.077
Keywords
Matrix; Nephronectin; ERK; Gene expression
Funding
- Heart and Stroke Foundation of Ontario [NA 6282, CI 5958]
- National Sciences and Engineering Research Council of Canada [227937-01]
- Canadian Breast Cancer Research Alliance
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We found that nephronectin was significantly down-regulated by TGF-beta 1. To determine the function of nephronectin in osteogenesis, we generated various constructs to produce stable MC3T3-E1 cell lines, expressing and secreting nephronectin protein, including full-length (Npnt), lacking EGF-like repeats (Np-MAM), and lacking RGD and MAM domains (Np-EGF). We demonstrated that nephronectin promotes differentiation during osteoblast differentiation and the EGF-like repeats were essential. Lack of these repeats resulted in inhibiting the change in morphology. Over-expression of nephronectin results in earlier formation of bone nodules than the vector control. ERK activation is essential for nephronectin-induced osteoblast differentiation. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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