Journal
FEBS LETTERS
Volume 583, Issue 17, Pages 2819-2826Publisher
WILEY
DOI: 10.1016/j.febslet.2009.07.036
Keywords
RNA ligase ribozyme; DSL ribozyme; Trans-acting ribozyme; Modular engineering; Multiple-turnover; Loop-receptor interaction
Funding
- Ministry of Education, Science, Sports and Culture, Japan
- JST
- Japan Society for the Promotion of Science Research Fellowships
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We previously developed a synthetic cis-acting RNA ligase ribozyme with 3'-5' joining activity termed DSL'' (designed and selected ligase). DSL was easily transformed into a trans-acting form because of its highly modular architecture. In this study, we investigated the modular properties and turnover capabilities of a trans-acting DSL, tDSL-1/GUAA. tDSL-1/GUAA exhibited remarkably high activity compared with the parental cis-acting DSL, and it attained a high turnover number. Taken together, the results indicate that a loop-receptor interaction plays a significant role in determining the activity of the trans-acting ribozyme and in its ability to perform multiple turnovers of the reaction. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
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