Journal
FEBS LETTERS
Volume 583, Issue 18, Pages 3033-3038Publisher
WILEY
DOI: 10.1016/j.febslet.2009.08.010
Keywords
Autolysin; Peptidoglycan; O-acetylation; Enterococcus faecalis
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Autolysins are potentially lethal enzymes that partially hydrolyze peptidoglycan for incorporation of new precursors and septum cleavage after cell division. Here, we explored the impact of peptidoglycan O-acetylation on the enzymatic activities of Enterococcus faecalis major autolysins, the N-acetylglucosaminidase AtlA and the N-acetylmuramidase AtlB. We constructed isogenic strains with various O-acetylation levels and used them as substrates to assay E. faecalis autolysin activities. Peptidoglycan O-acetylation had a marginal inhibitory impact on the activities of these enzymes. In contrast, removal of cell wall glycopolymers increased the AtlB activity (37-fold), suggesting that these polymers negatively control the activity of this enzyme. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
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