Journal
FEBS LETTERS
Volume 582, Issue 27, Pages 3739-3743Publisher
WILEY
DOI: 10.1016/j.febslet.2008.09.054
Keywords
Glycosynthase; 1,2-alpha-L-fucosidase; 2 '-fucosyllactose; Inverting alpha-glycosidase
Funding
- Protein 3000 project of the Ministry of Education, Culture, Sport, Science, and Technology of Japan
- Program for Promotion of Basic Research Activities for Innovative Biosciences (PROBRAIN)
- 21st Century COE Program
- Institute for Virus Research, Kyoto University
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Fucosyloligosaccharides have great therapeutic potential. Here we present a new route for synthesizing a Fuc alpha 1,2-Gal linkage by introducing glycosynthase technology into 1,2-alpha-L-fucosidase. The enzyme adopts a unique reaction mechanism, in which asparagine-423 activated by aspartic acid-766 acts as a base while asparagine-421 fixes both a catalytic water and glutamic acid-566 ( an acid) in the proper orientations. Glycosynthase activity of N421G, N423G, and D766G mutants was examined using beta-fucosyl fluoride and lactose, and among them, the D766G mutant most effectively synthesized 2'-fucosyllactose. 1,2-alpha-L-Fucosynthase is the first glycosynthase derived from an inverting alpha-glycosidase and from a glycosidase with an unusual reaction mechanism. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
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