Journal
FEBS LETTERS
Volume 582, Issue 16, Pages 2435-2440Publisher
WILEY
DOI: 10.1016/j.febslet.2008.06.007
Keywords
very long-chain fatty acid; 3-hydroxyacyl-CoA dehydratase; lipid; ELOVL; HACD
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Very long-chain fatty acids are produced through a four-step cycle. However, the 3-hydroxyacyl-CoA dehydratase catalyzing the third step in mammals has remained unidentified. Mammals have four candidates, HACD1-4, based on sequence similarities to the recently identified yeast Phs1, although HACD3 and HACD4 share relatively weak similarity. We demonstrate that all four of these human proteins are indeed 3-hydroxyacyl-CoA dehydratases, in growth suppression experiments using a PHS1-shut off yeast strain and/or in vitro 3-hydroxypalmitoyl-CoA dehydratase assays. HACD proteins exhibit distinct tissue-expression patterns. We also establish that HACD proteins interact with the condensation enzymes ELOVL1-7, with some preferences. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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