Journal
FEBS LETTERS
Volume 582, Issue 29, Pages 4047-4052Publisher
WILEY
DOI: 10.1016/j.febslet.2008.10.046
Keywords
Double-stranded RNA; RNA silencing suppressor; Virus; Inhibitor
Funding
- utilizing advanced technologies in agriculture, forestry and fisheries [1753]
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To find out whether we can control plant virus diseases by blocking viral RNA silencing suppressors (RSSs), we developed a strategy to screen inhibitors that block the association of RSSs with siRNAs using a surface plasmon resonance assay. The screened chemicals were tested in competition with RSSs for binding to siRNAs using a mobility shift assay. We then confirmed that tested chemicals actually inhibited the RSS activity in vivo using a protoplast assay which was developed for this purpose. This entire system can be adapted to screening inhibitors of not only plant viruses but also some animal viruses possessing RSSs. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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