Journal
FEBS LETTERS
Volume 582, Issue 29, Pages 4032-4038Publisher
WILEY
DOI: 10.1016/j.febslet.2008.10.045
Keywords
Isoprenoid; Yeast; 2-Methyl erythritol 4-phosphate; Mevalonate; Saccharomyces cerevisiae
Funding
- Firmenich SA
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A eukaryotic mevalonate pathway transferred and expressed in Escherichia coli, and a mammalian hydrocortisone biosynthetic pathway rebuilt in Saccharomyces cerevisiae are examples showing that transferring metabolic pathways from one organism to another can have a powerful impact on cell properties. In this study, we reconstructed the E. coli isoprenoid biosynthetic pathway in S. cerevisiae. Genes encoding the seven enzymatic steps of the pathway were cloned and expressed in S. cerevisiae. mRNA from the seven genes was detected, and the pathway was shown able to sustain growth of yeast in conditions of inhibition of its constitutive isoprenoid biosynthetic pathway. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
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