Journal
FEBS LETTERS
Volume 582, Issue 16, Pages 2458-2462Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2008.06.012
Keywords
channel; molecular dynamics; nitrogen; Arabidopsis
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Aquaporins and/or aquaglyceroporins regulate the permeability of plant membranes to water and small, uncharged molecules. Using molecular simulations with a plant plasma membrane aquaporin tetramer, the residues in the channel constriction region were identified as the crucial determinants of ammonia and urea conductance. The impact of these residues was experimentally verified using AtPIP2; 1 pore mutants. Several, but not all, mutants with a NIP-like selectivity filter promoted yeast growth on urea or ammonia as sole sources of nitrogen. TIP-like mutants conducted urea but not NH3, and a residue without direct contact to the pore lumen was critical for conduction in the mutants. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
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