Journal
FEBS LETTERS
Volume 583, Issue 1, Pages 219-223Publisher
WILEY
DOI: 10.1016/j.febslet.2008.12.006
Keywords
Chlorophyllide a reductase; Superoxide generation; Heterologous expression; Growth cessation; Rhodobacter sphaeroides; Synechocystis sp PCC6803
Funding
- 21C Frontier Microbial Genomics and Applications Center Program
- Ministry of Education, Science and Technology, Korea
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The photosynthetic growth of Synechocystis sp. PCC6803 ceased upon expression of Rhodobacter sphaeroides chlorophyllide a reductase (COR). However, an increase in cytosolic superoxide dismutase level in the recombinant Synechocystis sp. PCC6803 completely reversed the growth cessation. This demonstrates that COR generates superoxide in Synechocystis sp. PCC6803. Considering the dissolved oxygen (DO) level suitable for COR, the intracellular DO of this oxygenic photosynthetic cell appears to be low enough to support COR-mediated superoxide generation. The growth arrest of Synechocystis sp. PCC6803 by COR may give an insight into the evolutionary path from bacteriochlorophyll a biosynthetic pathway to chlorophyll a, which bypasses COR reaction. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
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