Journal
FEBS LETTERS
Volume 582, Issue 23-24, Pages 3270-3274Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2008.08.036
Keywords
GPIb-IX complex; Juxtamembrane region; Polybasic residue cluster; Protein biosynthesis; Trafficking
Funding
- National Institutes of Health [HL082808]
- American Heart Association [0565078Y]
- Harry S. and Isabel C. Cameron Foundation Fellowship
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Platelet glycoprotein (GP) Ib-IX complex requires all its three subunits for efficient expression on the cell surface, but the underlying molecular basis is not fully clear. Using transfected Chinese hamster ovary cells as the model system, we demonstrate that juxtamembrane residues 149-154 in the cytoplasmic domain of the GPIb beta subunit is required for assembly and surface expression of the GPIb-IX complex. The complex, or GPIbb by itself, lacking these residues is retained in the endoplasmic reticulum. Our results thus have illustrated an important role of the GPIbb cytoplasmic domain in biosynthesis of the GPIb-IX complex.
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