Journal
FEBS LETTERS
Volume 582, Issue 15, Pages 2173-2177Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2008.04.065
Keywords
Wig-1; p53; dsRNA; RNA Helicase A; hnRNP A2/B1
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The p53-induced Wig-1 gene encodes a double stranded RNA-binding zinc finger protein. We generated Saos-2 osteosarcoma cells expressing tetracycline-inducible Flag-tagged human Wig-1. Induction of Wig-1 expression by doxycycline inhibited cell growth in a long-term assay but did not cause any changes in cell cycle distribution nor increased fraction of apoptotic cells. Using co-immunoprecipitation and mass spectrometry, we identified two Wig-l-binding proteins, hnRNP A2/B1 and RNA Helicase A, both of which are involved in RNA processing. The binding was dependent on the presence of RNA. Our results establish a link between the p53 tumor suppressor and RNA processing via hnRNPA2/B1 and RNA Helicase A.
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