Journal
FEBS LETTERS
Volume 582, Issue 30, Pages 4147-4152Publisher
WILEY
DOI: 10.1016/j.febslet.2008.11.016
Keywords
UCP3; ROS; Aging; Mitochondria; 4-HNE; Mild uncoupling
Funding
- The Netherlands Organization for Health Research & Development (ZonMw) [9120.6050]
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Uncoupling protein 3 (UCP3) is suggested to protect mitochondria against aging and lipid-induced damage, possibly via modulation of reactive oxygen species (ROS) production. Here we show that mice overexpressing UCP3 (UCP3Tg) have a blunted age-induced increase in ROS production, assessed by electron spin resonance spectroscopy, but only after addition of 4-hydroxynonenal (4-HNE). Mitochondrial function, assessed by respirometry, on glycolytic substrate was lower in UCP3Tg mice compared to wild types, whereas this tended to be higher on fatty acids. State 4o respiration was higher in UCP3Tg animals. To conclude, UCP3 overexpression leads to increased state 4o respiration and, in presence of 4-HNE, blunts the age-induced increase in ROS production. (c) 2008 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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