Journal
FEBS JOURNAL
Volume 286, Issue 4, Pages 642-652Publisher
WILEY
DOI: 10.1111/febs.14666
Keywords
gene regulation; microRNA target; microRNA target validation; miRISC
Categories
Funding
- Natural Sciences and Engineering Research Council
- Canadian Institutes of Health Research
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As an important regulator of gene expression in eukaryotes, microRNAs (miRNAs) have been subject to extensive research to understand their pleiotropic roles in development and pathological processes. Here, we review strategies that have been developed to map microRNA-target interactions and the most effective methods to attribute post-transcriptional gene regulation through miRNA-mediated silencing. We further discuss the importance of the biological context for silencing.
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