Journal
FEBS JOURNAL
Volume 280, Issue 13, Pages 3058-3068Publisher
WILEY
DOI: 10.1111/febs.12245
Keywords
biocatalysis; cofactor regeneration; hydrogen; hydrogenase; NADH; oxidoreductase; oxygen tolerance; Ralstoniaeutropha; redox reactions
Categories
Funding
- ERC [297503, 258600]
- Deutsche Forschungsgemeinschaft (DFG through the Cluster of Excellence 'Unifying Concepts in Catalysis', Berlin
- Clara Immerwahr Award from UniCat
- European Research Council (ERC) [258600, 297503] Funding Source: European Research Council (ERC)
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A large number of industrially relevant enzymes depend upon nicotinamide cofactors, which are too expensive to be added in stoichiometric amounts. Existing NAD(P)H-recycling systems suffer from low activity, or the generation of side products. H2-driven cofactor regeneration has the advantage of 100% atom efficiency and the use of H2 as a cheap reducing agent, in a world where sustainable energy carriers are increasingly attractive. The state of development of H2-driven cofactor-recycling systems and examples of their integration with enzyme reactions are summarized in this article. The O2-tolerant NAD+-reducing hydrogenase from Ralstoniaeutropha is a particularly attractive candidate for this approach, and we therefore discuss its catalytic properties that are relevant for technical applications.
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