Journal
FEBS JOURNAL
Volume 278, Issue 19, Pages 3739-3747Publisher
WILEY-BLACKWELL
DOI: 10.1111/j.1742-4658.2011.08291.x
Keywords
activation volume; hammerhead ribozyme; high pressure; kinetics; RNA
Categories
Funding
- Centre National de la Recherche Scientifique (CNRS)
- UMPC University Paris
- Centre National de la Recherche Scientifique
- Centre National d'Etudes Spatiales (CNES)
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The activity of the full-length hammerhead ribozyme requires a tertiary interaction between its distal loops leading to the closure of the molecule and its stabilization in the active conformation. In this study, the conformational changes accompanying the cis-cleavage reaction of Chrysanthemum chlorotic mottle viroid hammerhead ribozyme were investigated by high-pressure experiments on the complete cleavage reaction. Two activation volumes (Delta V(not equal)) were measured, pointing to the presence of two different populations of molecules corresponding to fast-cleaving and slowcleaving ribozymes in the reaction mixture. The fast population, with a small Delta V(not equal), of 2.6 mL.mol(-1), most likely represents molecules in the near-active conformation, whereas the slow population, with a larger Delta V(not equal), of 11.6 mL.mol(-1), represents molecules that need a larger conformational change to induce activity. In addition, pH-dependence experiments suggest that the group whose deprotonation is required for activity intervenes in the formation of the transition state or in the chemistry of the reaction, but not in the conformational change that precedes it.
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