Journal
FEBS JOURNAL
Volume 277, Issue 18, Pages 3769-3779Publisher
WILEY
DOI: 10.1111/j.1742-4658.2010.07778.x
Keywords
data analysis; database; enzyme; kinetics; metadata
Categories
Funding
- EPSRC
- BBSRC
- BBSRC/EPSRC [BB/C008219/1]
- Klaus Tschira Foundation (KTF)
- German Federal Ministry of Education and Research (BMBF)
- Biotechnology and Biological Sciences Research Council [BB/E016065/1] Funding Source: researchfish
- BBSRC [BB/E016065/1] Funding Source: UKRI
Ask authors/readers for more resources
A limited number of publicly available resources provide access to enzyme kinetic parameters. These have been compiled through manual data mining of published papers, not from the original, raw experimental data from which the parameters were calculated. This is largely due to the lack of software or standards to support the capture, analysis, storage and dissemination of such experimental data. Introduced here is an integrative system to manage experimental enzyme kinetics data from instrument to browser. The approach is based on two interrelated databases: the existing SABIO-RK database, containing kinetic data and corresponding metadata, and the newly introduced experimental raw data repository, MeMo-RK. Both systems are publicly available by web browser and web service interfaces and are configurable to ensure privacy of unpublished data. Users of this system are provided with the ability to view both kinetic parameters and the experimental raw data from which they are calculated, providing increased confidence in the data. A data analysis and submission tool, the kineticswizard, has been developed to allow the experimentalist to perform data collection, analysis and submission to both data resources. The system is designed to be extensible, allowing integration with other manufacturer instruments covering a range of analytical techniques.
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