Journal
FASEB JOURNAL
Volume 33, Issue 1, Pages 1188-1198Publisher
FEDERATION AMER SOC EXP BIOL
DOI: 10.1096/fj.201800784R
Keywords
CRISPR; Cas9 nuclease; knockout; primordial germ cell
Categories
Funding
- Korean Rural Development Administration, Cooperative Research Program for Agriculture Science and Technology Development [PJ01334801]
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Chicken as a food source is one of the most widespread domestic animals, and it has been used extensively as a research model. The clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 (Cas9) system is the most efficient and reliable tool for precise genome-targeted modification and has generated considerable excitement for industrial applications, as well as biologic science. Unlike in mammals, germline-transmittable primordial germ cells (PGCs) in chicken were used as an alternative strategy for the production of genetically altered chickens. Here, by combining the CRISPR-Cas9 platform and germ cell-mediated germline transmission, we generated G(0)/G(1) switch gene 2 (G0S2) knockout (KO) chickens, and G0S2 null KO chickens showed a dramatic reduction of abdominal fat deposition without affecting other economic traits. Additionally, G0S2 null KO chickens had altered fatty acid compositions in their blood and abdominal fat compared with wild-type chickens under normal dietary conditions. The global mRNA sequencing data showed that G0S2 disruption in chickens would activate the adipose tissue-specific peroxisomal oxidation pathway, and enoyl-coenzyme A (CoA), hydratase/3-hydroxyacyl CoA dehydrogenase might be a target molecule in metabolic homeostasis in the chicken adipose tissue. Our results demonstrate that the CRISPR-Cas9 system with chicken PGCs can facilitate the production of specific genome-edited chickens for practical applications, as well as basic research.Park, T. S., Park, J., Lee, J. H., Park, J.-W., Park, B.-C. Disruption of G(0)/G(1) switch gene 2 (G0S2) reduced abdominal fat deposition and altered fatty acid composition in chicken.
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