Journal
FASEB JOURNAL
Volume 27, Issue 10, Pages 4244-4253Publisher
FEDERATION AMER SOC EXP BIOL
DOI: 10.1096/fj.13-230441
Keywords
pericytes; angiogenesis; vascular development; immune cell infiltrate
Categories
Funding
- Wellcome Trust [088407/Z/09/Z, 081172/Z/06/Z, 098291/Z/12/Z]
- Deutsche Forschungsgemeinschaft
- European Research Council
- Arthritis Research-UK [19207]
- Cancer Research UK [16354, 13034] Funding Source: researchfish
- Medical Research Council [G0501974] Funding Source: researchfish
- Versus Arthritis [19207] Funding Source: researchfish
- MRC [G0501974] Funding Source: UKRI
- Wellcome Trust [088407/Z/09/Z, 081172/Z/06/Z] Funding Source: Wellcome Trust
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Junctional adhesion molecule C (JAM-C) is a transmembrane protein with significant roles in regulation of endothelial cell (EC) functions, including immune cell recruitment and angiogenesis. As these responses are important in promoting tumor growth, the role of EC JAM-C in tumor development was investigated using the ID8 syngeneic model of ovarian cancer. Within 10-15 wk, intraperitoneally injected ID8 cells form multiple tumor deposits and ascites that resemble human high-grade serous ovarian cancer. Compared to wild-type mice, survival in this model was increased in EC JAM-C knockouts (KOs; 88 vs. 96 d, P=0.04) and reduced in EC JAM-C transgenics (88 vs. 78.5 d, P=0.03), mice deficient in or overexpressing EC JAM-C, respectively. While tumor growth was significantly reduced in EC JAM-C KOs (87% inhibition at 10 wk, P<0.0005), this was not associated with alterations in tumor vessel density or immune cell infiltration. However, tumor microvessels from EC JAM-C-deficient mice exhibited reduced pericyte coverage and increased vascular leakage, suggesting a role for EC JAM-C in the development of functional tumor vessels. These findings provide evidence for a role for EC JAM-C in tumor growth and aggressiveness as well as recruitment of pericytes to newly formed blood vessels in a model of ovarian cancer.Leinster, D. A., Colom, B., Whiteford, J. R., Ennis, D. P., Lockley, M., McNeish, I. A., Aurrand-Lions, M., Chavakis, T., Imhof, B. A., Balkwill, F. R., Nourshargh, S. Endothelial cell junctional adhesion molecule C plays a key role in the development of tumors in a murine model of ovarian cancer.
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