4.7 Article

Role of the acidic N′ region of cardiac troponin I in regulating myocardial function

Journal

FASEB JOURNAL
Volume 22, Issue 4, Pages 1246-1257

Publisher

FEDERATION AMER SOC EXP BIOL
DOI: 10.1096/fj.07-9458com

Keywords

muscle; transgenic mouse model; contractility; heart

Funding

  1. NHLBI NIH HHS [P50 HL077101-010005, P50 HL077101, HL-83334, HL-60546, P50 HL074728-010004, P50 HL074728, P50 HL052318, P01 HL069779-06A17077, R01 HL061638-06, HL-52318, P01 HL022619-200019, P01 HL022619, R01 HL056370-04, HL-69799, HL-56370, P01 HL041496-090007, R01 HL061638, R01 HL056370, P01 HL069779-01, P01 HL069779] Funding Source: Medline

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Cardiac troponin I (cTnI) phosphorylation modulates myocardial contractility and relaxation during beta-adrenergic stimulation. cTnI differs from the skeletal isoform in that it has a cardiac specific N' extension of 32 residues (N' extension). The role of the acidic N' region in modulating cardiac contractility has not been fully defined. To test the hypothesis that the acidic N' region of cTnI helps regulate myocardial function, we generated cardiac-specific transgenic mice in which residues 2-11 (cTnI(Delta 2-11)) were deleted. The hearts displayed significantly decreased contraction and relaxation under basal and P-adrenergic stress compared to nontransgenic hearts, with a reduction in maximal Ca2+-dependent force and maximal Ca2+- activated Mg2+-ATPase activity. However, Ca2+ sensitivity of force development and cTnI-Ser(23/24) phosphorylation were not affected. Chemical shift mapping shows that both cTnI and cTnI(Delta 2-11) interact with the N lobe of cardiac troponin C (cTnC) and that phosphorylation at Ser(23/24) weakens these interactions. These observations suggest that residues 2-11 of cTnI, comprising the acidic N' region, do not play a direct role in the calcium-induced transition in the cardiac regulatory or N lobe of cTnC. We hypothesized that phosphorylation at Ser(23/24) induces a large conformational change positioning the conserved acidic N region to compete with actin for the inhibitory region of cTnI. Consistent with this hypothesis, deletion of the conserved acidic N' region results in a decrease in myocardial contractility in the cTnI(Delta 2-11) mice demonstrating the importance of acidic N' region in regulating myocardial contractility and mediating the response of the heart to beta-AR stimulation.

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