Journal
FASEB JOURNAL
Volume 22, Issue 9, Pages 3135-3145Publisher
FEDERATION AMER SOC EXP BIOL
DOI: 10.1096/fj.08-107086
Keywords
oxidative metabolism; mitochondria; diabetes; single nucleotide polymorphism; gene-environment interaction
Categories
Funding
- UK Medical Research Council
- Wellcome Trust
- Commission of the European Communities [LSHM-CT-2004-005272]
- Danish National Research Foundation [DG 02-512-555]
- Swedish Diabetes Association
- Swedish Heart-Lung Foundation
- Vasterbottens Health Authority
- Swedish Parkinson's Society
- SSF Genome Canada
- MRC [MC_U106188470] Funding Source: UKRI
- Medical Research Council [MC_U106188470] Funding Source: researchfish
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The purpose of this study was to characterize associations between PINK1 genotypes, PINK1 transcript levels, and metabolic phenotypes in healthy adults and those with type 2 diabetes (T2D). We measured PINK1 skeletal muscle transcript levels and 8 independent PINK1 single nucleotide polymorphisms (SNPs) in a cohort of 208 Danish whites and in a cohort of 1701 British whites (SNPs and metabolic phenotypes only). Furthermore, we assessed the effects of PINK1 transcript ablation in primary adipocytes using RNA interference (RNAi). Six PINK1 SNPs were associated with PINK1 transcript levels (P < 0.04 to P < 0.0001). Obesity modified the association between PINK1 transcript levels and T2D risk (interaction P=0.005); transcript levels were inversely related with T2D in obese (n=105) [odds ratio (OR) per SD increase in expression levels=0.44; 95% confidence interval (CI): 0.23, 0.84; P=0.013] but not in nonobese (n=103) (OR=1.20; 95% CI: 0.82, 1.76; P=0.34) individuals. In the British cohort, several PINK1 SNPs were associated with plasma nonesterified fatty acid concentrations. Nominal genotype associations were also observed for fasting glucose, 2-h glucose, and maximal oxygen consumption, although these were not statistically significant after correcting for multiple testing. In primary adipocytes, Pink1 knockdown affected fatty acid binding protein 4 (Fabp4) expression, indicating that PINK1 may influence substrate metabolism. We demonstrate that PINK1 polymorphisms are associated with PINK1 transcript levels and measures of fatty acid metabolism in a concordant manner, whereas our RNAi data imply that PINK1 may indirectly influence lipid metabolism.
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