Journal
FARADAY DISCUSSIONS
Volume 149, Issue -, Pages 291-299Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c005397j
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Funding
- Biotechnology and Biological Sciences Research Council [BB/C511572/1] Funding Source: Medline
- Engineering and Physical Sciences Research Council [EP/E032745/1, EP/E000584/1, EP/E032745/2, EP/F005407/1] Funding Source: researchfish
- EPSRC [EP/E032745/2, EP/F005407/1, EP/E000584/1, EP/E032745/1] Funding Source: UKRI
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DNA functionalised nanoparticle probes offer new opportunities in analyte detection. Ultrasensitive, molecularly specific targeting of analytes is possible through the use of metallic nanoparticles and their ability to generate a surface enhanced Raman scattering (SERS) response. This is leading to a new range of diagnostic clinical probes based on SERS detection. Our approaches have shown how such probes can detect specific DNA sequences by using a biomolecular recognition event to 'turn on' a SERS response through a controlled assembly process of the DNA functionalised nanoparticles. Further, we have prepared DNA aptamer functionalised SERS probes and demonstrated how introduction of a protein target can change the aggregation state of the nanoparticles in a dose-dependant manner. These approaches are being used as methods to detect biomolecules that indicate a specific disease being present with a view to improving disease management.
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