4.2 Article

Microbial diversity in Tunisian geothermal springs as detected by molecular and culture-based approaches

Journal

EXTREMOPHILES
Volume 14, Issue 6, Pages 501-514

Publisher

SPRINGER JAPAN KK
DOI: 10.1007/s00792-010-0327-2

Keywords

Archaea; Biodiversity; Ecology; Phylogeny; Physiology of thermophiles; Thermophile ecology; Systematics

Funding

  1. Ministry of Higher Education and Scientific Research in Tunisia
  2. CNRS (Centre National de la Recherche Scientifique)

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Prokaryotic diversities of 12 geothermal hot springs located in Northern, Central and Southern Tunisia were investigated by culture-based and molecular approaches Enrichment cultures tor both aerobic and anaerobic microorganisms were successfully obtained at temperatures ranging from 50 to 75 degrees C Fourteen strains including four novel species were cultivated and assigned to the phyla Firmicutes (9), Thermotogae (2), Betaproteobacterta (1), Synergistetes (1) and Bacteroidetes (1) Archaeal or universal oligonucleotide primer sets were used to generate 16S rRNA gene libraries Representative groups Included Proteobacteria, Firmicutes, Deinococcus-Thermus, Thermotogae, Synergistetes, Bacteroidetes, Aquificae Chloroflex candidate division OP9 in addition to other yet unclassified strains The archaeal library showed a low diversity of clone sequences belonging to the phyla Euryarchaeota and Crenarchaeota Furthermore, we confirmed the occurrence of sulfate reducers and methanogens by amplification and sequencing of dissimilatory sulfite reductase (dsrAB) and methyl coenzyme M reductase alpha-subunit (mcrA) genes Altogether, we discuss the diverse prokaryotic communities arising from the 12 geothermal hot springs studied and relate these findings to the physico-chemical features of the hot springs

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