Journal
EXTREMOPHILES
Volume 14, Issue 1, Pages 33-39Publisher
SPRINGER JAPAN KK
DOI: 10.1007/s00792-009-0285-8
Keywords
Long-chain alkane degradation; Aldehyde dehydrogenase; Geobacillus thermoleovorans; (Extreme) thermophilic microorganisms and their enzymology; Biochemical characterisation; Biodegradation of pollutants; Biotechnology of thermophiles; Enzymology; Gene cloning and expression; Isolation and characterization; Thermophiles and thermophilic enzymes
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Funding
- Program for Promotion of Basic Research Activities for Innovative Biosciences (PROBRAIN)
- NEDO
- KAKENHI [19380189]
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Geobacillus thermoleovorans B23 is capable of degrading long-chain alkanes at 70A degrees C. Bt-aldh, an aldehyde dehydrogenase gene in B23, was located in the upstream region of p21 whose expression level was dramatically increased when alkane degradation was started (Kato et al. 2009, BMC Microbiol 9:60). Like p21, transcription level of Bt-aldh was also increased upon alkane degradation. Bt-Aldh (497 aa, MW = 53,886) was overproduced in Escherichia coli, purified, and characterized biochemically. Bt-Aldh acted as an octamer, required NAD(+) as a coenzyme, and showed high activity against aliphatic long-chain aldehydes such as tetradecanal. The optimum condition for activity was 50-55A degrees C and pH 10.0. The activity was elevated to two- to threefold in the presence of 2 mM Ba2+, Ca2+, or Sr2+, while Mg2+ and Zn2+ inhibited the enzyme activity. Bt-Aldh represents thermophilic aldehyde dehydrogenases responsible for degradation of long-chain alkanes.
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