Journal
EXTREMOPHILES
Volume 13, Issue 4, Pages 643-656Publisher
SPRINGER JAPAN KK
DOI: 10.1007/s00792-009-0247-1
Keywords
Halophile; Extreme hypersaline; BIOLOG; Solar saltern; Salinity tolerance; Substrate usage
Categories
Funding
- NSF [0511888]
- California State University Long Beach
- SCAC
- Biological Sciences department mini-grant
- NASA
- Direct For Biological Sciences
- Div Of Biological Infrastructure [0511888] Funding Source: National Science Foundation
Ask authors/readers for more resources
Solar salterns are extreme hypersaline environments that are five to ten times saltier than seawater (150-300 g L-1 salt concentration) and typically contain high numbers of halophiles adapted to tolerate such extreme hypersalinity. Thirty-five halophile cultures of both Bacteria and Archaea were isolated from the Exportadora de Sal saltworks in Guerrero Negro, Baja California, Mexico. 16S rRNA sequence analysis showed that these cultured isolates included members belonging to the Halorubrum, Haloarcula, Halomonas, Halovibrio, Salicola, and Salinibacter genera and what may represent a new archaeal genus. For the first time, metabolic substrate usage of halophile isolates was evaluated using the non-colorimetric BIOLOG Phenotype MicroArray (TM) plates. Unique carbon substrate usage profiles were observed, even for closely related Halorubrum species, with bacterial isolates using more substrates than archaeal cultures. Characterization of these isolates also included morphology and pigmentation analyses, as well as salinity tolerance over a range of 50-300 g L-1 salt concentration. Salinity optima varied between 50 and 250 g L-1 and doubling times varied between 1 and 12 h.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available